The oral microbiota of patients with recurrent aphthous stomatitis

Journal of Oral Microbiology

²  Fei Sjöberg¹  Sofia Östman³  Maria Bankvall⁴  Agnes Wold¹  Mats Jontell⁴ 

[1] Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden;le Gita;Institute of Biomedicine, the Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden;Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Sweden

关键词: Oral mucosa;    oral mucosal disease;    oral bacteria;    Terminal-Restriction Fragment Length Polymorphism;    oral ulceration;    oral cavity;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Specificpathogenicbacteriahavebeenimplicatedinrecurrentaphthousstomatitis(RAS),achronicinflammatoryconditioncharacterisedbyulcerationsintheoralmucosa.However,theaetiologybehindthisconditionstillremainsunclear.Objective:ThebuccalmicrobiotaofpatientswithRASwascomparedtothatofcontrolsubjectstoinvestigateitspotentialroleforthiscondition.Design:Buccalswabswereobtainedfromnon-ulcerativeareasof60patients,ofwhom42patientshadlesionsatthetimeofsampling,and60healthyage-andgender-matchedcontrols.BacterialDNAwasextractedandanalysedbyTerminal-RestrictionFragmentLengthPolymorphism,usingenzymaticdigestionofthepolymerasechainreaction-amplified16SrRNAgene,yieldingaseriesofpeaks,eachrepresentingabacterialtaxon.Results:Twopeaks,60and489,weremoreprevalentinpatientswithRASthancontrols.Conversely,peaks58and490werelesscommoninpatientsthancontrols.Whenthepatientsweredividedintosubgroups,wefoundthattheobserveddifferencesinpeak-patternwererelatedtothepresenceoflesionsduringsampling.Conclusions:Themicrobiotaofthenon-inflamedbuccalmucosadifferedbetweenpatientsandcontrols.Thedifferencesweremostpronouncedinpatientswhopresentedwithlesionsduringsampling,suggestingthatadisturbanceinthenormalbuccalmicrobiotatriggersthepresenceoflesionsorthatpresenceoflesionsaltersthemicrobiota.Keywords:oralmucosa;oralmucosaldisease;oralbacteria;Terminal-RestrictionFragmentLengthPolymorphism;oralulceration;oralcavity(Published:29October2014)Citation:JournalofOralMicrobiology2014,6:25739-http://dx.doi.org/10.3402/jom.v6.25739Toaccessthesupplementarymaterialforthisarticle,pleaseseeSupplementaryfilesunderArticleToolsonline

【 授权许可】

Unknown