Synthesis of macromolecules in early mouse embryos cultured in vitro: RNA, DNA, and a polysaccharide component☆

Developmental Biology

Lajos Pikó¹ 

[1] Developmental Biology Laboratory, Veterans Administration Hospital, Sepulveda, California, USA

关键词: tRNA;    transfer RNA;    rRNA;    ribosomal RNA;    r-pre-RNA;    ribosomal precursor RNA;    dRNA;    RNA with DNA-like base composition;    mRNA;    messenger RNA;    HnRNA;    high-molecular-weight heterodisperse nuclear RNA;   

DOI  :  10.1016/0012-1606(70)90071-0

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theproductsofincorporationofuridine-5-3Hinembryosfromthe8-cellstagetothehatchedblastocyst(day5)wereanalyzedbysedimentationinsucrosegradients.RibosomalRNAisamajorproductofRNAsynthesisduringthisperiod.HeterodisperseRNA,includingagiant-sizelabileRNA,and4SRNAarealsosynthesized.Pulselabelingandactinomycinchaseexperimentshaveledtotheidentificationofarapidlylabeled39SRNAspeciesasaprecursorof28Sand18SrRNA.LowconcentrationsofdactinomycininhibitrRNAsynthesisselectivelyandtoanextentproportionatetodose.Uniformlylabeledthymidine-14CisincorporatedintoDNA,RNA,andpolysaccharide.ThesecomponentshavebeenseparatedandanalyzedinCsClandethidiumbromide-CsCldensitygradients.TheDNAsynthesizedisnuclearandlabeledtoaveryhighspecificactivity.TheevidencesuggeststhatnoextensivereplicationofmitochondrialDNAtakesplaceduringcleavage.Amajorportionofuniformlylabeledthymidine-14Cisincorporatedintoapolysaccharidecomponentduringtheblastocyststage(day4–5).Somepropertiesofthispolysaccharidearedescribed.

【 授权许可】

Unknown