Targeting of tail‐anchored proteins to yeast mitochondria in vivo

FEBS Letters

Egan, Billie²  George, Rebecca²  Isenmann, Sandra¹  Wattenberg, Binks¹  Beilharz, Traude²  Gratzer, Sabine²  Lithgow, Trevor² 

[1] Department of Human Immunology, Hanson Centre for Cancer Research, Box 14 Rundle Mall Post Office, Adelaide 5000, Australia;Department of Biochemistry, La Trobe University, Bundoora 3083, Australia

关键词: Tail anchor;    Mitochondrion;    Membrane protein;    Transmembrane domain;    Protein translocation;    mRNA;    messenger RNA;    rRNA;    ribosomal RNA;    tRNA;    transfer RNA;    F1β-;    the β-subunit of the F1F0-ATPase;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    GFP;    green fluorescent protein;   

DOI  :  10.1016/S0014-5793(99)00581-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tail-anchored proteins are inserted into intracellular membranes via a C-terminal transmembrane domain. The topology of the protein is such that insertion must occur post-translationally, since the insertion sequence is not available for membrane insertion until after translation of the tail-anchored polypeptide is completed. Here, we show that the targeting information in one such tail-anchored protein, translocase in the outer mitochondrial membrane 22, is contained in a short region flanking the transmembrane domain. An equivalent region is sufficient to specify the localisation of Bcl2 and SNARE proteins to the secretory membranes. We discuss the targeting process for directing members of this protein family to the secretory and mitochondrial membranes in vivo.

【 授权许可】

Unknown