Microinjection of the Monoclonal Anti-Tubulin Antibody YL1/2 Inhibits Cleavage of Sand Dollar Eggs

Cell Structure and Function

Takao Arai¹  Yukihisa Hamaguchi²  Mikako T. Oka² 

[1] Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo;Biological Laboratory, Faculty of Science, Tokyo Institute of Technology

关键词: cleavage;    microinjection;    mitotic apparatus;    monoclonal antibody;    sand dollar;    tubulin;   

DOI  :  10.1247/csf.15.373

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(21)Cited-By(2)Two monoclonal antibodies against a-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeasterjaponicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunobloting to react specifically with egg tubulin and by immunofluorescence to stain the mitotic apparatus [OKA et al., (1990). Cell Motil. Cytoskel. 16: 239-250]. Injection of YL1/2 prevented chromosome movement and cleavage, although the cleavage furrow developed in some cases. In all eggs injected at prometaphase, metaphase, or anaphase, the birefringence of the mitotic apparatus disappeared immediately after injection. Injection of D2D6had no significant effect on mitosis or cleavage of whole eggs injected after nuclear disappearance, although it prevented the disappearance of the nuclear envelope in 54% of the eggs injected before the disappearance. FITC-conjugated D2D6did not accumulate in the spindle when injected into the dividing sand dollar egg. These results indicate that YL1/2 disassembled microtubules, whereas D2D6 did not bind to microtubules in the living cell.

【 授权许可】

Unknown