Cleavage of DNA by polyamide-seco-CBI conjugates

DNA;cleavage;polyamide-seco-CBI conjugates

Chang, Aileen Yulin ; Dervan, Peter B.

University:California Institute of Technology

Department:Chemistry and Chemical Engineering

关键词: DNA;    cleavage;    polyamide-seco-CBI conjugates;   

Others  :  https://thesis.library.caltech.edu/8137/1/Chang%202001.pdf

美国|英语

来源: Caltech THESIS

【 摘 要 】

Small molecules that bind to any predetermined DNA sequence in the human genome are potentially useful tools for molecular biology and human medicine. Polyamides containing N-methylimidazole (Im) N-methylpyrrole (Py) are cell permeable small molecules that bind DNA according to a set of "pairing rules" with affinities and specificities similar to many naturally occurring DNA binding proteins. Py-Im polyamides offer a general approach to the chemical regulation of gene expression. We demonstrate here that polyamide containing a DNA alkylating moiety seco-CBI can specifically direct sequence specific DNA alkylation. We can also control the strand of DNA that is alkylated, depending on the enantiomer of seco-CBI used and the orientation of the polyamide relative to the alkylation site (Chapter 2). This class of molecules has been applied to a gene repair system in collaboration with the Baltimore group at Caltech (Chapter 3). Also reported are additional seco-CBI polyamide conjugates synthesized to study other systems (HIV-1 and COX-2) (Appendix 1).