Displacement of the Mitotic Apparatus Which Induces Ectopic Polar Body Formation or Parthenogenetic Cleavage in Starfish Oocytes

Developmental Biology

Yukihisa Hamaguchi¹ 

[1] Functional Bioengineering, Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo, 152-8551, Japan

关键词: centrosome;    meiosis;    micromanipulation;    mitotic apparatus;    oocyte;    polar body;    starfish;   

DOI  :  10.1006/dbio.2001.0421

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Whenthemitoticapparatus(MA)atmeiosisIandIIinstarfishoocyteswasdetachedfromtheanimalpoleandtranslocatedtotheothercortex,MAinducedpolarbodyformation,whichindicatesreattachmentofMAtothecortex.MAattachmentwassostrongthatMAatmeiosisIIwasfrequentlybrokenintotwopartsduringdetachmentandfromtheremnantpartremainingatthecortexanasterderivedandanucleusderivedfromthedetachedpart.Whentheywereapartuntilthecleavagestage,theoocytedividedintotheaster-containingandnucleus-containingblastomeresand,further,onlytheformerblastomeredividedrepeatedly.Thisresultindicatesthatthecentrosomeintheperipheralaster,whichpresumestobediscardedintothesecondpolarbody,alwayshasthecapacityofduplicationbutthecentrosomeintheinneraster,whichstaysintheoocyteinterior,hasnotthecapacityandconfirmsourpreviousreport(17Dev.Biol.203,62–74).Furthermore,itisfoundbyobservingmeioticMAformationthatthispeculiarcentrosomedeliveryatmeiosisIIisensuredbythefactthattheattachmentoftheasterstayingintheoocyteinteriortothecortexoccursearlierthancentrosomeduplication.

【 授权许可】

Unknown