Microtubule Patterning during Meiotic Maturation in Mouse Oocytes Is Determined by Cell Cycle-Specific Sorting and Redistribution of γ-Tubulin

Developmental Biology

David F. Albertini¹  Catherine M.H. Combelles¹ 

[1] Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, 02111

关键词: γ-tubulin;    centrosome;    spindle;    nuclear lamina;    oocyte;    meiosis;    nuclear;    cytoplasmic maturation;   

DOI  :  10.1006/dbio.2001.0444

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thetopographyofmicrotubuleassemblyeventsduringmeioticmaturationofanimaloocytesdemandstightspatialcontrolandtemporalprecision.Tobetterunderstandwhatregulatesthetimingandlocationofmicrotubuleassembly,synchronouslymaturingmouseoocyteswereevaluatedwithrespecttoγ-tubulin,pericentrin,andtotaltubulinpolymerfractionsatspecificstagesofmeioticprogression.γ-Tubulinremainedassociatedwithcytoplasmiccentrosomesthroughdiakinesisofmeiosis-1.Followingchromatincondensationandperinuclearcentrosomeaggregation,γ-tubulinrelocatedtoanuclearlamina-boundedcompartmentinwhichmeiosis-1spindleassemblyoccurred.γ-Tubulinwasstablyassociatedwiththemeioticspindlefromprometaphase-1throughtoanaphase-2,butalsoexhibitedcellcycle-specificrelocalizationtocytoplasmiccentrosomes.Specifically,anaphaseonsetofbothmeiosis-1and-2wascharacterizedbytheconcomitantappearanceofγ-tubulinandmicrotubulenucleationinsubcorticalcentrosomes.Briefpulsesoftaxolappliedatspecificcellcyclestagesenhanceddetectionofγ-tubulincompartmentalization,consistentwithaγ-tubulinlocalization-dependentspatialrestrictionofmicrotubuleassemblyduringmeioticprogression.Inaddition,ataxolpulseduringmeioticresumptionimpairedsubsequentγ-tubulinsorting,resultinginmonopolarspindleformationandcellcyclearrestinmeiosis-1;despitecellcyclearrest,polarbodyextrusionoccurredroughlyonschedule.Therefore,sortingofγ-tubulinisinvolvedinboththetimingoflocationofmeioticspindleassemblyaswellasthecoordinationofkaryokinesisandcytokinesisinmouseoocytes.

【 授权许可】

Unknown