Change in the Heterogeneous Distribution of Tubulin Isotypes in Mitotic Microtubules of the Sea Urchin Egg by Treatment with Microtubule Depolymerizing or Stabilizing Drugs.

Cell Structure and Function

Takao Arai¹  Yukihisa Hamaguchi²  Mikako T. Oka² 

[1] Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo;Biological Laboratory, Faculty of Science, Tokyo Institute of Technology

关键词: sea urchin egg;    microtubule heterogeneity;    mitotic apparatus;    tubulin isotypes;    hexylene glycol;    colcemid;   

DOI  :  10.1247/csf.16.125

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(31)Cited-By(5)In the mitotic sea urchin egg, the spindle microtubules were composed of different tubulin isotypes from those of astral microtubules using monoclonal antibodies [Oka et al. (1990) Cell Motil. Cytoskeleton, 16, 239-250]. Three of the antibodies, D2D6, DM1B, and YL1/2, were specific for spindle microtubules, astral microtubules and reactive with both microtubules, respectively. The mitotic sea urchin egg was treated with microtubule depolymerizing (colcemid and nocodazole) and stabilizing (hexylene glycol) drugs and change in the heterogeneous distribution of the tubulin isotypes was investigated by the immunofluorescence procedure using these three monoclonal anti-tubulin antibodies. We observed that: (1) the microtubule depolymerizing drugs caused quick depolymerization of most mitotic microtubules, and a small number of spindle microtubules remaining were stained with all three antibodies; (2) hexylene glycol induced many microtubules in the mitotic apparatus, which was stained with D2D6 but was not stained with DM1B; (3) hexylene glycol also induced a great number of miniasters in the cytoplasm, and they were stained with three antibodies. These results suggest that these drugs altered the distribution of tubulin isotypes in the mitotic microtubules during depolymerisation or polymerization within a short time.

【 授权许可】

Unknown