Method validation and stability study of quercetin in topical emulsions

Química Nova

Jabor, José R.¹  Baracat, Marcela M.¹  Universidade Estadual de Londrina, Londrina, Brasil¹  Vicentini, Fabiana T. M. C.¹  Verri Jr, Waldiceu A.¹  Universidade de São Paulo, Ribeirão Preto, Brasil¹  Casagrande, Rúbia¹  Georgetti, Sandra R.¹  Fonseca, Maria J. V.¹  Rafael, Janice A.¹ 

关键词: quercetin;    HPLC;    validation.;   

DOI  :  10.1590/S0100-40422009000700041

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

【 授权许可】

Unknown