The Best Extraction Technique for Kaempferol and Quercetin Isolation from Guava Leaves (Psidium guajava)

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Batubara, I.^1 ; Suparto, I.H.^1,2 ; Wulandari, N.S.^1

Department of Chemistry, Bogor Agriculture University, Indonesia^1

Tropical Biopharmaca Research Center, Bogor Agricultural University, Indonesia^2

关键词: Artemia salina;    Extraction techniques;    Extraction time;    guava leaves;    Kaempferol;    Psidium guajava;    quercetin;    Total flavonoids;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012060/pdf
DOI  :  10.1088/1755-1315/58/1/012060

来源: IOP

【 摘 要 】

Guava leaves contain various compounds that have biological activity such as kaempferol and quercetin as anticancer. Twelve extraction techniques were performed to obtain the best extraction technique to isolate kaempferol and quercetin from the guava leaves. Toxicity of extracts was tested against Artemia salina larvae. All extracts were toxic (LC50value less than 1000 ppm) except extract of direct soxhletation on guava leaves, and extract of sonication and soxhletation using n-hexane. The extract with high content of total phenols and total flavonoids, low content of tannins, intense color of spot on thin layer chromatogram was selected for high performance liquid chromatography analysis. Direct sonication of guava leaves was chosen as the best extraction technique with kampferol and quercetin content of 0.02% and 2.15%, respectively. In addition to high content of kaempferol and quercetin, direct sonication was chosen due to the shortest extraction time, lesser impurities and high toxicity.