In vivo NMR study of yeast fermentative metabolism in the presence of ferric irons

Journal of biosciences

Annalisa Santucci¹  Claudia Bonechi²  Silvia Martini²  Daniela Braconi¹  Claudio Rossi²  Maso Ricci1 2² 

[1] Department of Molecular Biology, Università di Siena, Via Fiorentina, 1–53100 Siena, Italy$$;Department of Pharmaceutical and Applied Chemistry, University of Siena, Via Aldo Moro, 2–53100 Siena, Italy$$

关键词: Ethanol yield;    in vivo NMR;    iron metabolism;    mathematical modelling;    oxidative stress;    Saccharomyces cerevisiae;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Mathematical modelling analysis of experimental data, obtained with in vivo NMR spectroscopy and 13C-labelled substrates, allowed us to describe how the fermentative metabolism in Saccharomyces cerevisiae, taken as eukaryotic cell model, is influenced by stress factors. Experiments on cellular cultures subject to increasing concentrations of ferric ions were conducted in order to study the effect of oxidative stress on the dynamics of the fermentative process. The developed mathematical model was able to simulate the cellular activity, the metabolic yield and the main metabolic fluxes occurring during fermentation and to describe how these are modulated by the presence of ferric ions.

【 授权许可】

Unknown