Metabolic response to exogenous ethanol in yeast: An in vivo statistical total correlation NMR spectroscopy approach

Journal of biosciences

Claudia Bonechi¹  Silvia Martini¹  Anna Maria¹  Marianna Aggravi¹  Claudio Rossi¹  Maso Ricci1 2¹ 

[1] Department of Pharmaceutical and Applied Chemistry, University of Siena, Siena, Italy and Center for Colloid and Surface Science (CSGI), Via della Lastruccia 3, Sesto Fiorentino, FI, Italy$$

关键词: Exogenous stress;    in vivo NMR;    metabolomic;    Saccharomyces cerevisiae;    STOCSY;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

In vivo NMR spectroscopy, together with selectively 13C-labelled substrates and ‘statistical total correlation spectroscopy’ analysis (STOCSY), are valuable tools to collect and interpret the metabolic responses of a living organism to external stimuli. In this study, we applied this approach to evaluate the effects of increasing concentration of exogenous ethanol on the Saccharomyces cerevisiae fermentative metabolism. We show that the STOCSY analysis correctly identifies the different types of correlations among the enriched metabolites involved in the fermentation, and that these correlations are quite stable even in presence of a stressing factor such as the exogenous ethanol.

【 授权许可】

Unknown