Interaction of neuron‐specific K+‐Cl− cotransporter, KCC2, with brain‐type creatine kinase

FEBS Letters

Fukuda, Atsuo¹  Inoue, Koichi¹  Ueno, Shinya¹ 

[1] Department of Physiology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan

关键词: KCC2;    Creatine kinase;    Two-hybrid;    ATP;    Cl− homeostasis;    GABA;    γ-aminobutyric acid;    [Cl−]i;    concentration of intracellular Cl−;    E Cl;    Cl− equilibrium potential;    KCC;    K+-Cl− cotransporter;    CKB;    brain-type creatine kinase;    NKCC;    Na+-K+-Cl− cotransporter;    PCR;    polymerase chain reaction;    aa;    amino acids;    HA;    hemagglutinin;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;    DAPI;    4′;    6-diamidino-2-phenylindole;    CK;    creatine kinase;    CKM;    muscle-type creatine kinase;    PCr;    phosphocreatine;   

DOI  :  10.1016/S0014-5793(04)00328-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

γ-Aminobutyric acid, a major inhibitory neurotransmitter within the adult central nervous system, is also known to be excitatory at early developmental stages due to the elevated intracellular Cl⁻ concentration. This functional change is primarily attributable to a K⁺-Cl⁻ cotransporter, KCC2, the expression of which is developmentally regulated in neurons. However, little detail information is available concerning the intracellular regulation of KCC2 function. Here, we identify an interaction between KCC2 and brain-type creatine kinase by means of yeast two-hybrid screening. This interaction, which was also detected in cultured cells and brain extracts, might contribute to KCC2-mediated modulation of Cl⁻ homeostasis.

【 授权许可】

Unknown