Interaction of mitochondrial creatine kinase with model membranes A monolayer study

FEBS Letters

Nicolay, Klaas²  Wallimann, Theo¹  Rojo, Manuel¹  Demel, Rudy²  Eppenberger, Hans M.¹  Hovius, Ruud² 

[1] Institute for Cell Biology, Eidgenössische Technische Hochschule, 8093-Zürich, Switzerland;Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, 3584 CH Utrecht, The Netherlands

关键词: Creatine kinase;    Model membrane;    Protein—lipid interaction;    Mitochondrion;    CK;    creatine kinase;    BB-CK;    brain form of CK;    MM-CK;    muscle form of CK;    Mi-CK;    mitochondrial CK;    Cr;    creatine;    PCr;    creatine phosphate;    ADP;    adenosine diphosphate;    ATP;    adenosine triphosphate;    BME;    2-mercaptoethanol;    EDTA;    ethylenediamine tetraacetic acid;    EGTA;    ethyleneglycol bis-(2-aminoethylether)-N;    N;    N′N′-tetraacetic acid;    BSA;    bovine serum albumin;    HEPES;    2-[4-(2-hydroxyethyl)-1-piperazinyl] ethane sulfonic acid;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;    FPLC;    fast protein liquid chromatography;   

DOI  :  10.1016/0014-5793(91)80374-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of mitochondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi-CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligometric form of Mi-CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi-CK was able to interact equally well with both inner and outer mitochondrial membranes.

【 授权许可】

Unknown