Interleukin‐6 is a positive regulator of tumor necrosis factor α‐induced adipose‐related protein in 3T3‐L1 adipocytes

FEBS Letters

Fasshauer, Mathias²  Lossner, Ulrike²  Klier, Margit²  Kralisch, Susan²  Paschke, Ralf²  Bluher, Matthias²  Chambaut-Guérin, Anne-Marie³  Klein, Johannes¹ 

[1] University of Lübeck, Department of Internal Medicine I, 23538 Lübeck, Germany;University of Leipzig, Department of Internal Medicine III, 04103 Leipzig, Germany;UMR 7079 CNRS, Université Pierre et Marie Curie, Centre de Recherche Biomédicale des Cordeliers, 15 rue de l’Ecole de Médecine, 75270 Paris Cedex 06, France

关键词: Adipocyte;    Insulin resistance;    Interleukin-6;    Obesity;    Tumor necrosis factor α;    Tumor necrosis factor α-induced adipose-related protein;    IL-6;    interleukin-6;    IRS;    insulin receptor substrate;    Jak;    Janus kinase;    MAP;    mitogen-activated protein;    PI;    phosphatidylinositol;    TIARP;    tumor necrosis factor α-induced adipose-related protein;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/S0014-5793(04)00096-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tumor necrosis factor (TNF) α-induced adipose-related protein (TIARP) is a novel TNFα-stimulated protein in adipocytes. Besides TNFα, interleukin (IL)-6 has recently been shown to be another adipocytokine implicated in insulin resistance. Therefore, the impact of IL-6 on TIARP gene expression in 3T3-L1 adipocytes was determined by quantitative real-time reverse transcription-polymerase chain reaction. Interestingly, TIARP mRNA expression was stimulated up to 3.8-fold by IL-6 in a dose-dependent fashion with significant stimulation detectable at effector concentrations as low as 3 ng/ml and maximal effects seen at 100 ng/ml IL-6. Induction of TIARP mRNA by IL-6 was time-dependent with significant upregulation occurring as early as 2 h after effector addition and maximal effects observed at 4 h. In parallel, TIARP protein synthesis was upregulated with maximal effects seen after 8 h of IL-6 treatment. Furthermore, the Janus kinase 2 inhibitor AG490 decreased TIARP mRNA expression. The increase of TIARP mRNA could be reversed by withdrawal of IL-6 for 24 h. Furthermore, TIARP mRNA induction by IL-6 was also seen in brown adipocytes but not in muscle and liver cells. Taken together, these results show that TIARP is acutely regulated in adipose tissue not only by TNFα but also by IL-6 which has been shown to be another important cytokine implicated in the pathogenesis of insulin resistance.

【 授权许可】

Unknown