Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator‐activated receptor γ (+/−) mice independent of adipocyte size

FEBS Letters

Makino, Hideichi¹  Ogura, Takahiro¹  Kadowaki, Takashi²  Nishimiya, Tatsuya¹  Onuma, Hiroshi¹  Osawa, Haruhiko¹  Terauchi, Yasuo²  Kubota, Naoto²  Ochi, Masaaki¹  Tang, Yan¹ 

[1] Department of Laboratory Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan;Department of Metabolic Disease, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

关键词: Phosphodiesterase 3B;    Peroxisome proliferator-activated receptor γ;    Adipocyte;    Insulin;    Type 2 diabetes;    Insulin resistance;   

DOI  :  10.1016/S0014-5793(03)00339-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/−) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/−) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

【 授权许可】

Unknown