Activation of mouse phosphodiesterase 3B gene promoter by adipocyte differentiation in 3T3‐L1 cells

FEBS Letters

Makino, Hideichi²  Taira, Masato³  Yamada, Kazuya¹  Niiya, Toshiyuki²  Suzuki, Yoshifumi³  Onuma, Hiroshi²  Osawa, Haruhiko² 

[1] Department of Biochemistry, Fukui Medical University and CREST, Japan Science and Technology, Fukui, Japan;Department of Laboratory Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791-0295, Japan;Second Department of Chiba University School of Medicine, Chiba, Japan

关键词: Phosphodiesterase 3B;    Adipocyte;    Differentiation;    Transcription;    Promoter;    Insulin resistance;   

DOI  :  10.1016/S0014-5793(01)02807-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of phosphodiesterase (PDE) 3B reduces free fatty acid output from adipocytes. Induction of PDE3B gene expression by adipocyte differentiation could improve insulin resistance. To examine whether the PDE3B promoter is activated by this differentiation, the 5′ flanking sequence of the mouse PDE3B gene was isolated. The transcription initiation site was determined to be located 195 bp upstream of the translation start site. No putative binding site for peroxisome proliferator-activated receptor γ was found within 2 kb upstream of the transcription initiation site. This region had promoter activity, which was further activated on adipocyte differentiation in 3T3-L1 cells.

【 授权许可】

Unknown