Effects of Leptin on Lipid Metabolism and Gene Expression of Differentiation-Associated Growth Factors and Transcription Factors during Differentiation and Maturation of 3T3-L1 Preadipocytes

Endocrine Journal

Sung Jae SHIN²  Yang Hwan RYU¹  Yong KO¹  Chul Young LEE⁴  Won Kon KIM¹  Sang Chul LEE³  Moon Sung KANG¹  Kwang-Hee BAE³  Min Ho KIM¹ 

[1] Division of Biotechnology, College of Biosciences and Biotechnology, Korea University;Department of Medical Science, Seoul Clinical Laboratories;Translational Research Center, KRIBB;Regional Animal Industry Center, Jinju National University

关键词: Leptin;    IGF-I;    PPAR;    Adipocyte;    Differentiation;    Lipid;   

DOI  :  10.1507/endocrj.K08E-115

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(42)Cited-By(11)The present study was designed to determine the effects of leptin on lipid metabolism and gene expression during differentiation and maturation of the 3T3-L1 murine preadipocyte. The preadipocytes were induced to differentiate in a growth medium containing 10% calf serum and a hormonal cocktail for 2 days. The cells were next allowed to maturate for 14 days in the growth medium supplemented with 10 μg/ml insulin or 500 ng/ml insulin-like growth factor (IGF)-I in the absence or presence of supplemented leptin. Leptin, at a dose of 5 to 500 ng/ml, had no effect on proliferation of undifferentiated 3T3-L1 cells. However, leptin suppressed the insulin- or IGF-I-stimulated lipid accumulation and enhanced the release of glycerol, a measure of lipolysis, in a dose-dependent manner during and after the maturation of the cell. Moreover, leptin at a dose of 50 ng/ml inhibited IGF-I gene expression during the entire differentiation and maturation and also peroxisome proliferator activated receptor (PPAR)-γ expression during late maturation as monitored by semi-quantitative reverse transcription-polymerase chain reaction. However, leptin exerted no effect on the expression of transforming growth factor-β, CCAT/enhancer binding protein-α and PPAR-δ. Taken together, results suggest the anti-lipogenic and lipolytic effects of leptin in differentiating and mature adipocytes may have been partly mediated by suppressing the expression of PPAR-γ and IGF-I genes.

【 授权许可】

Unknown