Endocrine Journal | |
Effects of Leptin on Lipid Metabolism and Gene Expression of Differentiation-Associated Growth Factors and Transcription Factors during Differentiation and Maturation of 3T3-L1 Preadipocytes | |
Sung Jae SHIN2  Yang Hwan RYU1  Yong KO1  Chul Young LEE4  Won Kon KIM1  Sang Chul LEE3  Moon Sung KANG1  Kwang-Hee BAE3  Min Ho KIM1  | |
[1] Division of Biotechnology, College of Biosciences and Biotechnology, Korea University;Department of Medical Science, Seoul Clinical Laboratories;Translational Research Center, KRIBB;Regional Animal Industry Center, Jinju National University | |
关键词: Leptin; IGF-I; PPAR; Adipocyte; Differentiation; Lipid; | |
DOI : 10.1507/endocrj.K08E-115 | |
学科分类:内分泌与代谢学 | |
来源: Japan Endocrine Society | |
【 摘 要 】
References(42)Cited-By(11)The present study was designed to determine the effects of leptin on lipid metabolism and gene expression during differentiation and maturation of the 3T3-L1 murine preadipocyte. The preadipocytes were induced to differentiate in a growth medium containing 10% calf serum and a hormonal cocktail for 2 days. The cells were next allowed to maturate for 14 days in the growth medium supplemented with 10 μg/ml insulin or 500 ng/ml insulin-like growth factor (IGF)-I in the absence or presence of supplemented leptin. Leptin, at a dose of 5 to 500 ng/ml, had no effect on proliferation of undifferentiated 3T3-L1 cells. However, leptin suppressed the insulin- or IGF-I-stimulated lipid accumulation and enhanced the release of glycerol, a measure of lipolysis, in a dose-dependent manner during and after the maturation of the cell. Moreover, leptin at a dose of 50 ng/ml inhibited IGF-I gene expression during the entire differentiation and maturation and also peroxisome proliferator activated receptor (PPAR)-γ expression during late maturation as monitored by semi-quantitative reverse transcription-polymerase chain reaction. However, leptin exerted no effect on the expression of transforming growth factor-β, CCAT/enhancer binding protein-α and PPAR-δ. Taken together, results suggest the anti-lipogenic and lipolytic effects of leptin in differentiating and mature adipocytes may have been partly mediated by suppressing the expression of PPAR-γ and IGF-I genes.
【 授权许可】
Unknown
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