Apg‐2 has a chaperone‐like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas

FEBS Letters

Sakurai, Toshiharu¹  Nonoguchi, Kohsuke¹  Itoh, Katsuhiko¹  Higashitsuji, Hiroaki¹  Subjeck, John R²  Sumitomo, Yasuhiko¹  Kaneko, Yoshiyuki¹  Fujita, Jun¹  Gotoh, Kazuhisa¹ 

[1] Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan;Department of Cellular Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA

关键词: Hepatocellular carcinoma;    Hsp70;    Hsp110;    Apg-2;    Apg-1;    Chaperone;    HA;    hemagglutinin;    HCC;    hepatocellular carcinoma;    Hsp;    heat shock protein;    Ig;    immunoglobulin;    PBS;    phosphate-buffered saline;    Rb;    retinoblastoma gene product;   

DOI  :  10.1016/S0014-5793(04)00034-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70-1. Expression of the Hsp110 family members was further analyzed, and increased transcript levels of Hsp110 and Apg-2, but not Apg-1, were found in 12 and 14, respectively, of 18 HCCs. Immunohistochemical analysis demonstrated the overexpression of the proteins in tumor cells. Apg-2 had chaperone ability similar to Hsp110 in a thermal denaturation assay using luciferase, and showed anti-apoptotic activity. These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities.

【 授权许可】

Unknown