Gene expression profile linked to p53 status in hepatitis C virus‐related hepatocellular carcinoma

FEBS Letters

Yamada-Okabe, Hisafumi³  Takemoto, Norikazu²  Oka, Masaaki²  Hamamoto, Yoshihiko¹  Iizuka, Norio²  Nakayama, Hironobu³  Tangoku, Akira²  Okada, Toshimasa²  Tamesa, Takao²  Mori, Naohide²  Miyamoto, Takanobu¹  Hamada, Kenji³  Uchimura, Shunji¹ 

[1] Department of Computer Science and Systems Engineering, Faculty of Engineering, Yamaguchi University, 2-16-1 Tokiwadai, Ube, Yamaguchi 755-8611, Japan;Department of Surgery II, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan;Pharmaceutical Research Department 4, Kamakaura Research Laboratories, Chugai Pharmaceutical Co. Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan

关键词: p53;    DNA chip;    Gene expression;    Hepatocellular carcinoma;    Cancer;    HCC;    hepatocellular carcinoma;    HCV;    hepatitis C virus;    wt-p53;    wild-type p53;    mt-p53;    mutant-type p53;    Gene abbreviations used are from LocusLink;   

DOI  :  10.1016/S0014-5793(03)01345-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the role of p53 in 22 hepatitis C virus (HCV)-infected hepatocellular carcinomas (HCCs), we compared the gene expression profiles of HCCs with wild-type p53 (wt-p53) (n=17) and those with mutant-type p53 (mt-p53) (n=5) by oligonucleotide microarray analysis. Among 83 p53-related genes identified by a supervised learning method, 25 were underexpressed, and 58 were overexpressed in mt-p53 HCCs compared with wt-p53 HCCs. With a computer search, we identified consensus p53-binding sequences in the 3-kb region upstream of the translation initiation site in 59 of the 83 genes, suggesting that the in vivo p53-associated transcription system is very complicated. These data will provide additional insights into p53-related pathogenesis in HCV-infected HCC.

【 授权许可】

Unknown