Identification of the genes responsive to etoposide‐induced apoptosis: application of DNA chip technology

FEBS Letters

Wang, Yixin¹  Bian, Junhui¹  Rea, Thomas¹  Sun, Yi¹  Gray, Steve¹ 

[1] Department of Molecular Biology, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, 2800 Plymouth Road, Ann Arbor, MI 48105, USA

关键词: Apoptosis;    DNA chip;    Etoposide;    Gene expression;    p53;    GPX;    glutathione peroxidase;    HnRNP;    heterogeneous nuclear ribonucleoprotein core protein A1;    MT-2;    metallothionein-II;    ODC;    ornithine carboxylase;    PCNA;    proliferating cell nuclear antigen;    S100A2;    S100 calcium-binding protein A2;    TGFβ-RII;    transforming growth factor-β type II receptor;    TXN;    thioredoxin;   

DOI  :  10.1016/S0014-5793(99)00136-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of detecting 6591 unique human mRNA species. 12 (Nine induced and three repressed) of the etoposide-responsive genes were further studied by Northern analysis and an agreement rate of 92% was reached. Among the 12 genes studied, two (WAF1/p21 and PCNA) are known p53 regulatory genes, two (glutathione peroxidase and S100A2 calcium-binding protein) appear to be the novel p53 target genes and the others appear to be p53-independent. Based upon these findings, the signalling pathways that possibly mediate etoposide-induced apoptosis are proposed.

【 授权许可】

Unknown