FEBS Letters | |
Oxidative DNA damage by hyperglycemia‐related aldehydes and its marked enhancement by hydrogen peroxide | |
Mizutani, Mika1  Oikawa, Shinji1  Kawanishi, Shosuke1  Murata, Mariko1  Hiraku, Yusuke1  | |
[1] Department of Environmental and Molecular Medicine, Mie University School of Medicine, 2-174, Edobashi, Tsu, Mie 514-8507, Japan | |
关键词: Diabetes mellitus; Hyperglycemia; Oxidative DNA damage; Glyceraldehyde; 3-Deoxyglucosone; 3-DG; 3-deoxyglucosone; GA; glyceraldehyde; GA3P; glyceraldehyde 3-phosphate; MG; methylglyoxal; AGE; advanced glycated end-product; 8-oxodG; 8-oxo-7; 8-dihydro-2′-deoxyguanosine (also known as 8-hydroxy-2′-deoxyguanosine); DTPA; diethylenetriamine-N; N; N′; N″; N″-pentaacetic acid; HPLC-ECD; high performance liquid chromatography coupled with an electrochemical detector; SOD; superoxide dismutase; | |
DOI : 10.1016/S0014-5793(03)01129-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Increased risks of cancers and oxidative DNA damage have been observed in diabetic patients. Many endogenous aldehydes such as 3-deoxyglucosone and glyceraldehyde (GA) increase under hyperglycemic conditions. We showed that these aldehydes induced Cu(II)-mediated DNA damage, including 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) formation. GA had the strongest ability to damage DNA, and addition of low concentrations of H2O2 markedly enhanced the DNA damage. GA significantly increased 8-oxodG formation in human cultured cells (HL-60), and H2O2 enhanced it. We conclude that oxidative DNA damage by hyperglycemia-related aldehydes, especially GA, and marked enhancement of DNA damage by H2O2 may participate in diabetes-associated carcinogenesis.
【 授权许可】
Unknown
【 预 览 】
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