New biomarker evidence of oxidative DNA damage in patients with non‐insulin‐dependent diabetes mellitus

FEBS Letters

Tanaka, Tomoyuki³  Ochi, Hirotomo⁴  Pasternack, Amos⁵  Okada, Kunihiko³  Wirta, Ole⁵  Hiai, Hiroshi³  Alho, Hannu²  Leinonen, Janne¹  Lehtimäki, Terho⁷  Rantalaiho, Vappu⁵  Toyokuni, Shinya³  Laippala, Pekka⁶ 

[1] Laboratory of Neurobiology, Medical School, University of Tampere, Tampere, Finland;Department of Mental Health and Alcohol Research, National Public Health Institute, P.O. Box 719, 00101 Helsinki, Finland;Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan;Japan Institute for the Control of Aging, Fukuroi, Shizuoka, Japan;Department of Internal Medicine, Tampere University Hospital, Tampere, Finland;Tampere School of Public Health, University of Tampere and Tampere University Hospital, Tampere, Finland;Department of Medical Biochemistry, Medical School, University of Tampere, Tampere, Finland

关键词: Oxidative stress;    Oxidative DNA damage;    8-Hydroxy-2′-deoxyguanosine;    Diabetes mellitus;    non-insulin-dependent;   

DOI  :  10.1016/S0014-5793(97)01273-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. We have investigated oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus (NIDDM) by urinary 8-OHdG assessments. We determined the total urinary excretion of 8-OHdG from 24 h urine samples of 81 NIDDM patients 9 years after the initial diagnosis and of 100 non-diabetic control subjects matched for age and gender. The total 24 h urinary excretion of 8-OHdG was markedly higher in NIDDM patients than in control subjects (68.2±39.4 μg vs. 49.6±37.7 μg, P=0.001). High glycosylated hemoglobin was associated with a high level of urinary 8-OHdG. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in NIDDM patients.

【 授权许可】

Unknown