Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8‐hydroxy‐2′‐deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment

FEBS Letters

Erhola, Marina³  Kellokumpu-Lehtinen, Pirkko¹  Uchida, Koji⁴  Nieminen, Markku M³  Tanaka, Tomoyuki²  Ochi, Hirotomo⁵  Osawa, Toshihiko⁴  Okada, Kunihiko²  Hiai, Hiroshi²  Alho, Hannu⁶  Toyokuni, Shinya² 

[1] Department of Oncology, University of Tampere, Tampere, Finland;Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan;Department of Respiratory Medicine, University of Tampere, Tampere, Finland;Laboratory of Food and Biodynamics, Nagoya University School of Agriculture, Nagoya, Japan;Japan Institute for the Control of Aging, Fukuroi, Shizuoka, Japan;Department of Mental Health and Alcohol Research, National Public Health Institute, Helsinki, Finland

关键词: Oxidative DNA damage;    Lung cancer;    Radiotherapy;    Chemotherapy;    8-Hydroxy-2′-deoxyguanosine;    Monoclonal antibody;    8-OHdG;    8-hydroxy-2′-deoxyguanosine;    SCC;    small-cell carcinoma;    HPLC;    high performance liquid chromatography;    ELISA;    enzyme-linked immunosorbent assay;    Gy;    gray;    CR;    complete response;    PR;    partial response;    NC;    no change;    PD;    progressive disease;    ANOVA;    an analysis of variance;    LSD;    least significance difference;   

DOI  :  10.1016/S0014-5793(97)00523-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ratios of urinary 8-hydroxy-2′-deoxyguanosine to urinary creatinine (8-OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation. Urinary 8-OHdG/creatinine levels of lung cancer patients were evaluated by enzyme-linked immunosorbent assay using a monoclonal antibody N45.1 during radiotherapy and chemotherapy. An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (non-SCC) patients during the course of radiotherapy. SCC patients showed higher levels of urinary 8-OHdG/creatinine than the controls. Furthermore, SCC patients with complete or partial response to the chemotherapy showed a significant decrease in urinary 8-OHdG/creatinine while patients with no change or progressive disease showed an increase.

【 授权许可】

Unknown