Differential expression of ATPAF1 and ATPAF2 genes encoding F1‐ATPase assembly proteins in mouse tissues

FEBS Letters

Petruzzella, Vittoria¹  Pı́cková, Andrea²  Houštěk, Josef²  Paul, Jan² 

[1] Department of Medical Biochemistry and Medical Biology, University of Bari, Piazza Giulio Cesare, 70124 Bari, Italy;Institute of Physiology and Centre for Integrated Genomics, Academy of Sciences of the Czech Republic, Vı́deňská 1083, 142 20 Prague 4 - Krč, Czech Republic

关键词: Mitochondrial ATPase;    Assembly;    ATPAF1;    ATPAF2;    Gene expression;    Brown adipose tissue;    ATPase;    ATP synthase;    F1;    catalytic part of ATPase;    Atp11p (Atpaf1p);    F1-ATPase assembly factor 1;    Atp12p (Atpaf2p);    F1-ATPase assembly factor 2;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(03)00890-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Atp11p (Atpaf1p; F₁-ATPase assembly factor 1) and Atp12p (Atpaf2p; F₁-ATPase assembly factor 2) are proteins required for the assembly of β (F₁-β) and α (F₁-α) subunits into the mitochondrial ATPase. Here we report about 100 times lower levels of ATPAF1 and ATPAF2 transcripts in relation to the mRNA levels of F₁ -α and F₁ -β in a range of mouse tissues. Quantitative reverse-transcription polymerase chain reaction revealed nearly constant ATPAF1 expression in all tissues in both adult and 5-day-old mice (up to two-fold differences), indicating that ATPAF1 rather behaves like a maintenance gene. In contrast, ATPAF2 expression differed up to 30-fold in the tissues analysed. ATPAF2 tissue-specific expression was also found to correlate well with mRNA levels of both F₁ -α and F₁ -β (BAT≫kidney, liver>heart, brain>skeletal muscle), showing the highest mRNA level in the thermogenic, ATPase-poor brown adipose tissue, which is characterised by a 10-fold decrease in ATPase/respiratory chain stoichiometry relative to the other tissues.

【 授权许可】

Unknown