Titration of the binding sites for the oligomycin‐sensitivity conferring protein in beef heart submitochondrial particles

FEBS Letters

Satre, Michel¹  Vignais, Pierre V.¹  Dupuis, Alain¹ 

[1] Laboratoire de Biochimie (CNRS/ERA 903 et INSERM U. 191), Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, 85 X, 38041 Grenoble Cédex, France

关键词: Mitochondrial ATPase;    Oligomycin-sensitivity conferring protein;    Pi—ATP exchange;    ATP hydrolysis;    F1;    beef heart mitochondrial coupling factor;    Fo;    proton-conducting sector of the ATPase complex;    containing the oligomycin binding site;    OSCP;    oligomycin-sensitivity conferring protein;    A particles;    submitochondrial particles from beef heart isolated in the presence of ammonia;    ASUA particles;    A particles successively treated with Sephadex;    urea and ammonia;   

DOI  :  10.1016/0014-5793(83)80256-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding parameters of the oligomycin-sensitivity conferring protein (OSCP) in inside-out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin-sensitive ATP—P_i exchange, and the oligomycin-sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1±0.5 mol OSCP with K _d 1.7 nM.

【 授权许可】

Unknown