Hypertonic activation of a non‐selective cation conductance in HeLa cells and its contribution to cell volume regulation

FEBS Letters

Sabirov, Ravshan¹  Shimizu, Takahiro¹  Okada, Yasunobu¹  Wehner, Frank¹ 

[1] Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan

关键词: HeLa cell;    Volume regulation;    Cation channel;    Amiloride;    Gadolinium;    Flufenamate;   

DOI  :  10.1016/S0014-5793(03)00868-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In whole-cell recordings on single HeLa cells, the hypertonic activation of a cation conductance with a selectivity ratio P_Na:P_Li:P_K:P_Cs:P_NMDG:P_Ca:P_Cl of 1.00:0.86:0.84:0.56:0.10:0.07:0.15 was observed. This (non-selective) cation conductance was reduced to 59 and 30% of maximal stimulation by Gd³⁺ and flufenamate, respectively, but it was insensitive to amiloride (with each compound applied at 100 μmol/l). As was determined by the Coulter counter technique, the cation conductance was the main mechanism of regulatory volume increase (RVI) in HeLa cells. Whereas a significant contribution of Na⁺/H⁺ antiport was also detectable, Na⁺-K⁺-2Cl⁻ symport most likely did not contribute to RVI.

【 授权许可】

Unknown