Volume‐activated Cl− secretion and transepithelial vinblastine secretion mediated by P‐glycoprotein are not correlated in cultured human T84 intestinal epithelial layers

FEBS Letters

Hirst, B.H.¹  Simmons, N.L.¹  McEwan, G.T.A.¹  Hunter, J.¹ 

[1] Gastrointestinal Drug Delivery Research Centre and Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, Newcastle upon Tyne, NE2 4HH, UK

关键词: P-glycoprotein;    MDR;    Cl− secretion;    Volume regulation;    T84 cells;   

DOI  :  10.1016/0014-5793(92)80626-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl⁻ secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T₈₄ cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 μM 1,9-dideoxy-forskolin, volume-stimulated Cl⁻ secretion is unaffected. In contrast, 100 μM 4,4′-diisothiocyanostilbene-2,2′disulphonic acid (DIDS) inhibited the volume-stimulated Cl⁻ secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl⁻ secretion but maintained a normal level of secretory vinblastine flux.

【 授权许可】

Unknown