Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

FEBS Letters

Kobayashi, Kaoru¹  Kawamoto, Takeshi¹  Moore, Rick¹  Yoshinari, Kouichi¹  Negishi, Masahiko¹ 

[1] Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

关键词: Nuclear receptor CAR;    Phenobarbital;    Nuclear translocation;    Heat shock protein;    Protein phosphatase;    PB;    phenobarbital;    OA;    okadaic acid;    GR;    glucocorticoid receptor;    HSP90;    heat shock protein 90;    PP5;    protein phosphatase 5;    PP2A;    protein phosphatase 2A;    GA;    geldanamycin;    DTSSP;    3;    3′-dithiobis(sulfosuccinimidyl propionate);    TCPOBOP;    1;    4-bis[2-(3;    5-dichloropyridyloxy)]benzene;    RT-PCR;    reverse transcription-polymerase chain reaction;    2-ME;    2-mercaptoethanol;   

DOI  :  10.1016/S0014-5793(03)00720-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.

【 授权许可】

Unknown