Nuclear localization of liver FBPase isoenzyme in kidney and liver

FEBS Letters

Slebe, Juan C.¹  Yáñez, Alejandro J.¹  Bertinat, Romina¹  Concha, Ilona I.¹ 

[1] Instituto de Bioquı́mica, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile

关键词: Gluconeogenesis;    Glucose metabolism;    Fructose-1;    6-bisphosphatase;    Localization;    Nucleus;    Nuclear translocation;    FBPase;    fructose-1;    6-bisphosphatase;    PEPCK;    phosphoenolpyruvate carboxykinase;    GS;    glycogen synthase;    GK;    glucokinase;    PAGE;    polyacrylamide gel electrophoresis;    NLS;    nuclear localization sequence;   

DOI  :  10.1016/S0014-5793(03)00809-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nuclear localization has been observed for glycolytic enzymes but not for key gluconeogenic enzymes. We report our findings on the intracellular localization of liver FBPase in rat liver and kidney, the main organs in the endogenous glucose production. Immunofluorescence and confocal analysis revealed that FBPase was present in the cytosol and, unexpectedly, inside the nucleus of hepatocytes and proximal cells of the nephron. Additionally, FBPase was found in the plasma membrane area of adjacent hepatocytes where glycogen is synthesized and in the apical region of proximal kidney cells. This subcellular distribution in multiple compartments suggests the presence of different localization signals on FBPase for diverse metabolic functions.

【 授权许可】

Unknown