Study on the Anti-tumor Mechanisms of Heat Shock Protein 72/alpha-fetoprotein Epitope Peptide Complex Against Hepatocellular Carcinoma Cell Hepa1-6

2nd International Symposium on Application of Materials Science and Energy Materials

材料科学;能源学

Xu, Bing^1 ; Wang, Xiaoping^1 ; Lin, Huanping^1

School of Shaanxi, University of Chinese Medicine, Xian, China^1

关键词: Alphafetoprotein (AFP);    Anti-tumor immune;    Antitumor immunity;    Heat shock protein;    Heat shock protein 72(HSP72);    Hepatocellular carcinoma cell;    Peptide complex;    Protein complexes;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/490/6/062016/pdf
DOI  :  10.1088/1757-899X/490/6/062016

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Objective: Mice immunized with heat shock protein 72 (HSP72)-alpha-fetoprotein (AFP) epitope peptide complex were used to study whether the complex has specific anti-tumor immune effect against AFP tumor. Methods: Kunming mice were immunized subcutaneously with HSP72-AFP peptide complex, and mice were immunized with AFP polypeptide and HSP72, respectively. The serum IFN-γ levels in mice after immunization were detected by ELISA. The killing effect of lymphocytes on hepatic cancer Hepal-6 cells was detected by MTT assay. The immune effect of protein complex was evaluated by tumor burden test in mice. Results: The serum IFN-r levels and the killing effect of lymphocytes on Hepal-6 cells in HSP72-AFP peptide complex group were significantly higher than those in AFP polypeptide and HSP72 group (P