An electrochemical immunosensor for AFP measurement based on the magnetic Fe3O4@Au@CS nanomaterials

2018 International Conference on Advanced Materials, Intelligent Manufacturing and Automation

材料科学;机械制造;运输工程

Li, Shanshan^1 ; Liang, Jintao^1 ; Zhou, Zhide^1 ; Li, Guiyin^1

School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi

541004, China^1

关键词: Alphafetoprotein (AFP);    Current response;    Electrochemical immunosensors;    Horseradish peroxidase;    Linear relationships;    Measurement-based;    Specific recognition;    Ultra sensitives;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/382/2/022017/pdf
DOI  :  10.1088/1757-899X/382/2/022017

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

An ultrasensitive electrochemical immunosensor was fabricated based on the magnetic Fe3O4@Au@CS nanomaterials for alpha fetoprotein (AFP). First, the Fe3O4@Au@CS nanocomposites were prepared with Fe3O4@Au NPs as the core and chitosan (CS) as the shell. Then, The human AFP antibody (Ab1) was immobilized on Fe3O4@Au@CS as the capture probe, the mouse anti-human AFP antibody (Ab2) and horseradish peroxidase (HRP) were conjugated on the gold nanoparticles (Au NPs) as the detection probe. In the presence of AFP, the immunosensor Fe3O4@Au@CS-Ab1@AFP@Ab2-Au-HRP was formed via specific recognition of antigen and antibody. Due to the immobilized HRP catalyzed the decomposition of H2O2 resulting in a substantial current, the response signal of the sensor was recorded by the amperometric (i-t) method. The current response presented the linear relationship to the concentration of AFP, a dynamic range from 0.01 to 8 μg/mL, with the detection limit of 1.78 ng/mL.