In vitro DNA‐binding properties of VirB, the Shigella flexneri virulence regulatory protein

FEBS Letters

Beloin, Christophe¹  McKenna, Sorcha¹  Dorman, Charles J.¹ 

[1] Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland

关键词: VirB protein;    Transcription factor;    Gene regulation;    Leucine zipper;    Helix-turn-helix motif;    Shigella flexneri;   

DOI  :  10.1016/S0014-5793(03)00524-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNA-binding activity of the Shigella flexneri VirB transcription factor was studied in vitro. The protein was found to bind non-specifically to DNA, but showed preferential binding to VirB-dependent promoter sequences. DNA binding was contingent on the presence of an intact helix-turn-helix motif. While high molecular mass protein–DNA complexes were formed in both specific and non-specific interactions with DNA, mutant derivatives of VirB lacking a leucine zipper domain or a carboxyl-terminal-located oligomerisation domain formed discrete complexes, indicating that an ability to oligomerise on DNA was responsible for the formation of high molecular mass complexes by the wild-type protein.

【 授权许可】

Unknown