cDNA clones encoding leucine‐zipper proteins which interact with G‐CSF gene promoter element 1‐binding protein

FEBS Letters

Nishizawa, Mikio¹  Nagata, Shigekazu¹ 

[1] Osaka Bioscience Institute, 6-2-4 Furuedal, Suita-shi, Osaka 565, Japan

关键词: Granulocyte colony-stimulating factor;    Transcription factor;    Leucine zipper;    Protein-protein interaction;    Lipopolysaccharide;    G-CSF;    granulocyte colony-stimulating factor;    LPS;    lipopolysaccharide;   

DOI  :  10.1016/0014-5793(92)80094-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene expression of granulocyte colony-stimulating factor (G-CSF) is induced by lipopolysaccharide (LPS). GPE1, a cis-controlling element of the G-CSF gene, functions as an LPS-responsive element. GPE1-binding protein (GPE1-BP), a leucine-zipper protein, did not independently activate G-CSF gene expression. Protein blot analysis with biotinylated GPE1-BP revealed that there were nuclear proteins that interact specifically with GPE1-BP. Three leucine-zipper proteins were isolated from mouse cDNA expression libraries by this method: NF-IL6, ATF4, and a novel ATF4-related ATFx. The interactions of these proteins with GPE1-BP may play key roles in G-CSF gene expression.

【 授权许可】

Unknown