Molecular cloning of cDNA and a chromosomal gene encoding GPE1‐BP, a nuclear protein which binds to granulocyte colony‐stimulating factor promoter element 1

FEBS Letters

Nishizawa, Mikio¹  Nagata, Shigekazu¹  Wakabayashi-Ito, Noriko¹ 

[1] Osaka Bioscience Institute, 6-2-4 Furuedai, Suita-shi, Osaka 565, Japan

关键词: Granulocyte colony-stimulating factor;    Macrophage;    Lipopolysaccharide;    Leucine zipper;    C/EBP;    DNA-binding protein;   

DOI  :  10.1016/0014-5793(91)80452-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Granulocyte colony-stimulating factor (G-CSF) is produced from macrophages in response to lipopolysaccharide (LPS). GPE1, a cis-acting element of the G-CSF gene promoter, functioned as an LPS-inducible element. We isolated cDNA and a chromosomal gene encoding the mouse GPEI-binding protein (GPE1-BP). A 150-amino acid protein deduced from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the cDNA showed GPE1-binding activity. Transcripts were ubiquitously detected, and may be spliced from two exons of a single gene.

【 授权许可】

Unknown