Short interfering RNA‐directed inhibition of hepatitis B virus replication

FEBS Letters

Eguchi, Katsumi²  Matsumoto, Kojiro²  Nakao, Kazuhiko¹  Ishikawa, Hiroki²  Ichikawa, Tatsuki²  Hamasaki, Keisuke² 

[1] Health Research Center, Nagasaki University, Nagasaki 852-8501, Japan;The First Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan

关键词: Hepatitis B virus;    siRNA;    RNAi;    dsRNA;    double-stranded RNA;    HBeAg;    hepatitis B e antigen;    HBsAg;    hepatitis B surface antigen;    HBV;    hepatitis B virus;    RNAi;    RNA interference;    siRNA;    short interfering RNA;   

DOI  :  10.1016/S0014-5793(03)00400-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA interference (RNAi) is the process by which double-stranded RNA directs sequence-specific degradation of mRNA. In mammalian cells, RNAi can be triggered by 21-nucleotide duplexes of short interfering RNA (siRNA). We examined effects of siRNA on hepatitis B virus (HBV) replication. Human hepatoma cells were transfected with HBV DNA and siRNA against HBV-pregenome RNA. Transfection experiments demonstrated that the siRNA reduced the amount of HBV-pregenome RNA and resulted in reduction of the levels of replicative intermediates and viral protein. Our results indicate that siRNA-mediated gene silencing inhibits HBV replication through suppression of viral RNA, which may be useful as a potential therapeutic modality.

【 授权许可】

Unknown