Long‐lasting RNAi activity in mammalian neurons

FEBS Letters

Hohjoh, Hirohiko¹  Omi, Kazuya¹  Tokunaga, Katsushi² 

[1] National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan;Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

关键词: RNA interference;    Mammalian neuron;    Synthetic small interfering RNA;    Long double-stranded RNA;    Apoptosis;    RNAi;    RNA interference;    siRNA;    small interfering RNA;    dsRNA;    double-stranded RNA;    PKR;    interferon-inducible;    dsRNA-activated protein kinase;    nt;    nucleotide;   

DOI  :  10.1016/S0014-5793(04)00017-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21-nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long-lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence-specific RNAi activity involving long dsRNA appears to be masked.

【 授权许可】

Unknown