pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase

FEBS Letters

Fraga, Hugo¹  Esteves da Silva, Joaquim C.G.¹  Fontes, Rui² 

[1] LAQUIPAI, Chemistry Department, Faculdade de Ciências, Universidade do Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal;Biochemistry Department (U38-FCT), Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal

关键词: Adenosine(5′)tetraphospho(5′)adenosine;    Dehydroluciferin;    Dinucleoside polyphosphate;    Firefly luciferase;    Luciferin;    Ap4A;    adenosine(5′)tetraphospho(5′)adenosine;    L;    dehydroluciferin;    L-AMP;    dehydroluciferyl-adenylate;    LH2;    luciferin;    LH2-AMP;    luciferyl-adenylate;    PPase;    inorganic pyrophosphatase;    IP-HPLC;    ion pair high performance liquid chromatography;    RP-HPLC;    reverse phase HPLC;   

DOI  :  10.1016/S0014-5793(03)00382-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl-adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl-adenylate to the acceptor nucleotide.

【 授权许可】

Unknown