Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferase

FEBS Letters

Günther Sillero, Marı́a A.²  de Diego, Anabel²  Fontes, Rui¹  Ortiz, Begoña²  Sillero, Antonio² 

[1] Serviço de Quı́mica Fisiológica, Faculdade de Medicina, Universidade do Porto, Porto, Portugal;Instituto de Investigaciones Biomédicas Alberto Sols, CSIC, Facultad de Medicina, UAM, Arzobispo Morcillo 4, 28029 Madrid, Spain

关键词: Ap4A;    Luciferase;    Luciferin;    Dehydroluciferin;    Coenzyme A;    Ap4A;    adenosine(5′)tetraphospho(5′)adenosine;    L;    dehydroluciferin;    L-AMP;    dehydroluciferyl-adenylate;    Lase;    luciferase;    L-CoA;    dehydroluciferyl-coenzyme A;    LH2;    luciferin;    LH2-AMP;    luciferyl-adenylate;    P3;    tripolyphosphate;    p4A;    adenosine 5′-tetraphosphate;    PPase;    pyrophosphatase;   

DOI  :  10.1016/S0014-5793(98)01301-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was previously assumed that E·LH₂-AMP was the intermediate complex in the synthesis of Ap₄A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin (LH₂) was used as cofactor. However, here we show that LH₂ is partly transformed, shortly after the onset of the luciferase reaction, to dehydroluciferin (L) with formation of an E·L-AMP complex which is the main intermediate for the synthesis of Ap₄A. Formation of three more derivatives of LH₂ were also observed, related to the production of light by the enzyme. CoA, a known stimulator of light production, inhibits the synthesis of Ap₄A by reacting with the E·L-AMP complex and yielding L-CoA.

【 授权许可】

Unknown