Time course of luciferyl adenylate synthesis in the firefly luciferase reaction

FEBS Letters

Günther Sillero, Maria A¹  Dukhovich, Alexey¹  Sillero, Antonio¹ 

[1] Departamento de Bioquímica, Instituto de Investigaciones Biomedicas del CSIC, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain

关键词: Firefly luciferase;    Luciferin;    ATP;    Luciferyl adenylate;    Enzyme kinetics;    Ap4A;    diadenosine 5′;    5‴-p 1;    p 4-tetraphosphate or diadenosine tetraphosphate;    E;    luciferase;    LH2;    d-luciferin;    LH2-AMP;    luciferyl adenylate;    RLU;    relative light units;   

DOI  :  10.1016/0014-5793(96)01038-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed. The properties of luciferyl adenylate, enzymatically or chemically synthesized, as substrate of luciferase, have been compared. The potential use of luciferyl adenylate for luciferase detection is here proposed.

【 授权许可】

Unknown