Firefly luciferase synthesizes P1,P4‐bis(5'‐adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphates

FEBS Letters

Guranowski, Andrzej¹  Sillero, Maria A.Günther¹  Sillero, Antonio¹ 

[1] Departamento de Bioquímica, Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain

关键词: Diadenosine tetraphosphate;    Dinucleoside polyphosphate;    Firefly luciferase;    Pyrophosphate;    Synthetase;    Ap4A;    diadenosine 5';    5'''-P1;    P4-tetraphosphate or diadenosine tetraphosphate;    Ap4G;    adenosine 5'-P1-tetraphosphoP4-5'''-guanosine;    Gp4G;    diguanosine 5';    5'''-P1;    P4-tetraphosphate or diguanosine tetraphosphate;    Np4N;    dinucleoside tetraphosphate;    DNPP;    dinucleoside polyphosphate;    NMP;    nucleoside monophosphate;    X;    organic acid able to form an anhydride bond with NMP;   

DOI  :  10.1016/0014-5793(90)80409-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthesis of P¹,P⁴-bis(5'-adenosyl)tetraphosphate (Ap₄A) has been considered, for a long time, to be catalyzed mainly by some aminoacyl-tRNA synthetases [Brevet et al. (1989) Proc. Natl. Acad. Sci. USA 86, 8275-8279]. Recently, yeast Ap₄A phosphorylase, acting in reverse (Guranowski et al. (1988) Biochemistry 27, 2959-2964), was shown to synthesize Ap₄A, too. In the case of the synthetases, the intermediate complex E-aminoacyl-AMP may serve as donor of AMP to ATP, yielding Ap₄A. Here we demonstrate that firefly luciferase (EC 1.13.12.7) which forms the E-luciferin-AMP intermediate also synthesizes Ap₄A as well as other dinucleoside polyphosphates. We suggest moreover that: other enzymes (mainly synthetases and some transferases), which catalyze the transfer of a nucleotidyl moiety, via nucleotidyl-containing intermediates and releasing PP_i may produce dinucleoside polyphosphates.

【 授权许可】

Unknown