Dinucleoside tetraphosphatase from human blood cells

FEBS Letters

Costas, Maria Jesús²  Cameselle, José Carlos²  García-Agundez, José Augusto¹  Canales, José²  Fernández, Ascensión²  Pinto, Rosa Maria² 

[1] Departamento de Farmacologia y Psiquiatria, Facultad de Medicina, Universidad de Extremadura, E-06080 Badajoz, Spain;Departamento de Bioquimica y Biologia Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, E-06080 Badajoz, Spain

关键词: Diadenosine tetraphosphate;    Ap4A;    Dinucleoside tetraphosphatase;    Human blood;    Ap3A;    diadenosine 5';    5'''-P 1;    P 3-triphosphate;    Ap4A;    diadenosine 5 ';    5'''P 1;    P 4-tetraphosphate;    Np4N;    dinucleoside 5';    5 ' ' '-P1;    P 4-tetraphosphate;    Np4Nase;    dinueleoside tetraphosphatase (EC 3.6.1.17);   

DOI  :  10.1016/0014-5793(91)80021-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dinucleoside tetraphosphatase (Np4Nase; EC 3.6.1.17) has been purified 170000-fold from a 30–60% ammonium sulfate fraction of a human blood cell extract. Purification included a dye-ligand affinity elution step using the inhibitor adenosine 5'-tetraphosphate. Human blood Np4Nase resembled rat liver Np4Nase, including recognition by anti-rat Np4Nase, but differed from homogeneous human leukemia Np4Nase in the 1000-fold lower specific activity of the latter. The results are discussed in relation to the potential role of diadenosine tetraphosphate (Ap4A) in the control of cell division and the turnover of Ap4A in blood.

【 授权许可】

Unknown