Uridine 5′‐polyphosphates (p4U and p5U) and uridine(5′)polyphospho(5′)nucleosides (Up n Ns) can be synthesized by UTP:glucose‐1‐phosphate uridylyltransferase from Saccharomyces cerevisiae

FEBS Letters

de Diego, Anabel²  Guranowski, Andrzej¹  Sillero, Antonio²  Günther Sillero, Marı́a Antonia² 

[1] Katedra Biochemii i Biotechnologii, Akademia Rolnicza, ul. Wołyńska 35, 60-637 Poznań, Poland;Departamento de Bioquı́mica, Instituto de Investigaciones Biomédicas Alberto Sols, UAM/CSIC, Facultad de Medicina, c. Arzobispo Morcillo 4, 28029 Madrid, Spain

关键词: UTP:glucose-1-phosphate uridylyltransferase;    UDP-glucose;    Uridine polyphosphate;    Uridine-oligophospho-nucleoside;    Dinucleoside polyphosphate;    Saccharomyces cerevisiae;    Np n N′;    nucleoside(5′)polyphospho(5′)nucleoside;    P2;    pyrophosphate;    P3;    tripolyphosphate;    P4;    tetrapolyphosphate;    p4U;    uridine 5′-tetraphosphate;    Up4N;    uridine(5′)tetraphospho(5′)nucleoside;   

DOI  :  10.1016/S0014-5793(04)00126-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

UTP:glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) from Saccharomyces cerevisiae can transfer the uridylyl moiety from UDP-glucose onto tripolyphosphate (P₃), tetrapolyphosphate (P₄), nucleoside triphosphates (p₃Ns) and nucleoside 5′-polyphosphates (p₄Ns) forming uridine 5′-tetraphosphate (p₄U), uridine 5′-pentaphosphate (p₅U) and dinucleotides, such as Ap₄U, Cp₄U, Gp₄U, Up₄U, Ap₅U and Gp₅U. Unlike UDP-glucose, UDP-galactose was not a UMP donor and ADP was not a UMP acceptor. This is the first example of an enzyme that may be responsible for accumulation of dinucleoside tetraphosphates containing two pyrimidine nucleosides in vivo. Occurrence of such dinucleotides in S. cerevisiae and Escherichia coli has been previously reported (Coste et al., J. Biol. Chem. 262 (1987) 12096–12103).

【 授权许可】

Unknown