FBPase is in the nuclei of cardiomyocytes

FEBS Letters

Gizak, A¹  Dzugaj, A¹ 

[1] Department of Animal Physiology, Institute of Zoology, Wrocław University, Cybulskiego 30, 50-205 Wrocław, Poland

关键词: Fructose-1;    6-bisphosphatase;    Cardiomyocyte;    Nucleus;   

DOI  :  10.1016/S0014-5793(03)00183-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intracellular localization of FBPase in the cardiac muscle of the pig was studied by immunohistochemistry. In contrast to data from skeletal muscle [Gizak, A., Rakus, D. and Dzugaj, A. (2003) Histol. Histopathol. 18, 135–142], in cardiomyocytes FBPase was present not only in the cytoplasm, but surprisingly, also in the nucleus. Results of the microscopic investigation were confirmed by immunoblotting, measurement of FBPase activity in isolated cardiomyocyte nuclei and by determination of the nuclear FBPase I _0.5 toward adenosine monophosphate (AMP), which was the same as for the purified enzyme.

【 授权许可】

Unknown