Activation of chicken liver fructose‐1,6‐bisphosphatase by oxidized glutathione

FEBS Letters

Han, Peter¹  Johnson, Joe¹  Emmett, Nerimiah²  Han, Grace³  Hang, David¹ 

[1] Dolpus E. Milligan Science Research Institute, Atlanta University Center, Atlanta, GAUSA 30310;Morehouse School of Medicine, Atlanta, GA 30310, USA;Morehouse College, Atlanta, GA 30310, USA

关键词: Glutathione;    Enzyme activation;    Fructose-1;    6-bisphosphatase;    (Chicken liver);   

DOI  :  10.1016/0014-5793(86)81166-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of chicken liver fructose-1,6-bisphosphatase with oxidized glutathione (GSSG) leads to an increase in activity. This activation is markedly enhanced if treatment is performed in the presence of AMP or Mn²⁺. The effects of AMP and Mn²⁺ appear to be synergistic. The maximal activation is over 13-fold and is accompanied by the disappearance of 4 sulfhydryl groups per molecule of enzyme. Both fructose 1,6-bisphosphate and fructose 2,6-bisphosphate can largely prevent this activation. Activation can be reversed by dithiothreitol or cysteine. It appears that GSSG activates this enzyme by thiol/disulfide exchanges with the enzyme's specific sulfhydryl groups.

【 授权许可】

Unknown