Stimulation of spinach (Spinacia oleracea) chloroplast fructose‐1,6‐bisphosphatase by mercuric ions

FEBS Letters

Ashton, Anthony R¹  Siegel, Gabriele M¹ 

[1]Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, 95440 Bayreuth, Germany

关键词: Chloroplast enzyme;    Enzyme activation;    Fructose-1;    6-bisphosphatase;    Mercury;    Redox regulation;    Spinach;   

DOI  :  10.1016/S0014-5793(97)00385-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chloroplast fructose-1,6-bisphosphatase can exist in an active reduced form or a less active oxidised form. Oxidised fructose bisphosphatase from spinach (Spinacia oleracea) could be stimulated up to many hundred-fold by 0.1 mM HgCl₂ whereas fructose bisphosphatases from rabbit, yeast, a non-chloroplast enzyme from spinach and the reduced chloroplast enzyme were only inhibited by HgCl₂. Stimulation of the enzyme was maximal at pH 8.0 and low magnesium concentrations where the oxidised enzyme normally has little activity.

【 授权许可】

Unknown