Low affinity cholecystokinin receptor inhibits cholecystokinin‐ and bombesin‐induced oscillations of cytosolic Ca2+ concentration

FEBS Letters

Uhm, Dae-Yong¹  Lee, Kwang Kook¹  Park, Myoung Kyu¹ 

[1] Department of Physiology, Sungkyunkwan University School of Medicine, 300 Chunchundong, Janganku, Suwon 440-746, South Korea

关键词: Cholecystokinin;    Bombesin;    Caffeine;    Pancreatic acinar cell;    Cytosolic free Ca2+ concentration;    Low affinity cholecystokinin receptor;   

DOI  :  10.1016/S0014-5793(03)00165-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated whether low affinity cholecystokinin (CCK) receptors suppress agonist-induced rises of cytosolic free Ca²⁺ concentration ([Ca²⁺]c) in pancreatic acinar cells by using properties of caffeine. A high concentration of caffeine (20 mM) completely blocked inositol 1,4,5-trisphosphate (InsP₃)-induced [Ca²⁺]c rises but spared the InsP₃-independent long-lasting [Ca²⁺]c oscillations. In the presence of 20 mM caffeine, only high concentrations of CCK, but not bombesin or JMV-180, suppressed the caffeine-resistant CCK or bombesin-induced [Ca²⁺]c oscillations, indicating that low affinity CCK receptors inhibit agonist-induced [Ca²⁺]c oscillations. It could be one of the underlying mechanisms by which low affinity CCK receptors suppress secretion in pancreatic acinar cells.

【 授权许可】

Unknown