Thimerosal modulates the agonist‐specific cytosolic Ca2+ oscillatory patterns in single pancreatic acinar cells of mouse

FEBS Letters

Hoshina, Yuko¹  Kamimura, Noritaka¹  Wu, Jie¹  Takeo, Teruko¹  Wada, Junro¹  Wakui, Makoto¹  Sechiko, Suga¹ 

[1] Department of Physiology 1, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036, Japan

关键词: Ca2+ oscillation;    Inositol trisphosphate;    Thimerosal;    Pancreatic acinar cell;    Ca2+-dependent Cl− current;    Patch-clamp;   

DOI  :  10.1016/0014-5793(96)00646-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Modulation of the agonist-specific cytosolic Ca²⁺ oscillatory pattern by thimerosal has been investigated in single pancreatic acinar cells using patch-clamp perforated whole-cell recording to measure the calcium-dependent chloride current (I _C1(Ca²⁺₎). 1 μM thimerosal, which fails to evoke Ca²⁺ oscillation f alone, clearly changed the pattern of Ca²⁺ oscillation from pulsatile spikes (evoked by low concentrations of activators) to sinusoidal or transient oscillations. The mimetic action of thimerosal was independent of extracellular Ca²⁺, was blocked by extracellular application of dithiothreitol or 10 mM caffeine, as well as by internal perfusion with heparin; but was unaffected by ruthenium red. We conclude that thimerosal modulates the agonist-specific cytosolic Ca²⁺ oscillatory patterns mediated by sensitizing the InsP₃-induced Ca²⁺ release.

【 授权许可】

Unknown