Hormonal activation of single K+ channels via internal messenger in isolated pancreatic acinar cells

FEBS Letters

Suzuki, Kazuo¹  Petersen, Ole H.¹  Petersen, Carl C.H.¹ 

[1] MRC Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, PO Box 147, Brownlow Hill, Liverpool L69 3BX, England

关键词: Pancreatic acinar cell;    Single K+ channel;    Cholecystokinin;    Calcium dependency;   

DOI  :  10.1016/0014-5793(85)80131-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism underlying hormonal activation of potassium channels was investigated in pig pancreatic acinar cells by patch-clamp single-channel and whole-cell current recordings. It was shown directly that a peptide hormone belonging to the cholecystokinin-gastrin family, CCK5, can activate single voltage-sensitive potassium channels which can be blocked by tetraethylammonium. The single-channel currents were recorded from electrically isolated cell-attached membrane patches to which the hormone had no access and the activation must therefore involve an intracellular messenger. The hormonal response requires external Ca²⁺ in the isolated membrane-patch area indicating that calcium gating is not directly linked to hormone-receptor interaction.

【 授权许可】

Unknown