FEBS Letters | |
The human interleukin‐6 (IL‐6) receptor exists as a preformed dimer in the plasma membrane | |
Meinert, Werner1  Kallen, Karl-Josef1  Rose-John, Stefan1  Schuster, Björn1  | |
[1] Biochemisches Institut, Christian Albrechts Universität zu Kiel, Olshausenstr. 40, D-24098 Kiel, Germany | |
关键词: Interleukin-6; Cytokine receptor; Plasma membrane; Dimer; CBM; cytokine binding module; FNIII; fibronectin-type-III; IL-6; interleukin-6; IL-6R; IL-6 receptor; vIL-6; viral interleukin-6; | |
DOI : 10.1016/S0014-5793(03)00154-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The recently solved X-ray structure of the extracellular portion of the interleukin-6 (IL-6) receptor (IL-6R) revealed an IL-6R dimer in the crystal lattice which probably represents a physiological dimer. Performing coprecipitation experiments with two differently tagged IL-6R variants expressed in COS-7 cells, we show that an IL-6R dimer exists in the plasma membrane in the absence of IL-6. Ligand binding does not seem to affect the dimerization status. When lysates of COS-7 cells expressing only one of the IL-6R variants are mixed, spontaneous dimerization occurs. Thus, the IL-6R dimer observed in the crystal structure represents a physiologically occurring phenomenon.
【 授权许可】
Unknown
【 预 览 】
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